High-throughput Biology - From Sequence to Networks 2019 - Lab 6 Supplemental
Generating a preqc report for the E. coli data set
# First build an FM-index of the two E. coli Illumina data sets:
sga index -t 4 -a ropebwt ecoli.illumina.15x.fastq
sga index -t 4 -a ropebwt ecoli.illumina.50x.fastq
# Next, we can run `sga preqc` to run the calculations and generate the PDF report.
sga preqc -t 4 ecoli.illumina.15x.fastq > ecoli.illumina.15x.preqc
sga preqc -t 4 ecoli.illumina.50x.fastq > ecoli.illumina.50x.preqc
python sga-preqc-report.py ecoli.illumina.15x.preqc ecoli.illumina.50x.preqc