.005 mg/ml insulin, .01 mg/ml transferrin, 30nM sodium selenite, 10 nM hydrocortisone, 10 nM beta estradiol, 10 mM HEPES, 2 mM L-glutamine
0.005mg/ml insulin, 0.005mg/ml transferrin and 5ng/ml GM-CSF
0.005 mg/ml Insulin, 0.01 mg/ml transferrin, 30 nM sodium selenite, 10 nM hydrocortisone, 10 nM beta estradiol, 10 mM HEPES, 1 mM L-glutamine
0.5 ug/ml hydrocortisone + 10ug/ml insulin
0.02 mg/ml insulin, 0.01 mg/ml transferrin, 25 nM sodium selenite, 50 nM Hydrocortisone, 1 ng/ml EGF(don't filter), 0.01 mM ethanolamine, 0.01 mM phosphorylethanolamine, 100 pM triiodothyronine, 0.5% bovine serum albumin, 0.5 mM sodium pyruvate,  2mM L-glutamine, 10nM HEPES
.02 mg/ml insulin, .01 mg/ml transferrin, 25nM sodium selenite, 1ng/mL EGF,  50 nM hydrocortisone, 2 mM L-glutamine, 0.5 sodium pyruvate
.005 mg/ml insulin, .01 mg/ml transferrin, 25nM sodium selenite, 50nM hydrocortisone, 1 ng/mL epidermal growth factor, 0.01mM ethanolamine, 0.01mM phosphorylethanolamine, 100pM triiodothyronine, 0.5%(w/v) bovine serum albumin, 0.5 mM sodium pyruvate, 2mM L-glutamine
.005 mg/ml insulin, .01 mg/ml transferrin, 30nM sodium selenite, 10 nM hydrocortisone, 10 nM beta estradiol,  2 mM L-glutamine
4.5 g/L glucose, 1.5 g/L sodium bicarbonate
2mM L-GLUTAMINE + 0.4UG/ML HYDROCORTISONE
2mM L-Glutamine ; 0.4 ug/ml Hydrocortisone
2mM L-glutamine, Insulin, Transferrin, Selenite
10ug/ml bovine insulin; 16ug/ml glutathione
.02 mg/ml insulin, .01 mg/ml transferrin, 25nM sodium selenite, 50 nM hydrocortisone, 2 mM L-glutamine, 0.5 sodium pyruvate, 1ng/mL EGF
5ug/ml insulin, 10ug/ml transferrin, 3.0x10^-8 M Na Selenite (added 5ml Insulin-Transferrin-Selenium); 1.0x10^-8 M Hydrocortisone, 1.0x10^-8 beta estradiol, 2mM L-glutamine, 10mM HEPES
0.005 mg/mL insulin, 0.01 mg/mL transferrin, 30nM sodium selenite, 2mM L-glutamine
2mM L-glutamine, 0.5ug/ml hydrocortisone, 10ug/ml insulin
2mM L-Glutamine, 20 IU/L Bovine Insuline
4.5g/L glucose +1.5 g/Lsodium bicarbonate
5ug/ml insulin, 10ug/ml transferrin, 30nM Na Selenite, 10nM Hydrocortisone, 10nM Beta-estradiol, 10mM HEPES, 2mM L-glutamine
HEPES, ADENOSINE TRIPHOSPHATE, AMINO ACIDS
2mM L-GLUTAMINE, 4X MEM NON-ESSENTIAL AMINO ACIDS
2 mM L-Glutamine ; 1.5 g/L sodium bicarbonate; 0.1 mM NEAA; 1.0 mM sodium pyruvate
0.01 mg/ml insulin; 0.01 mg/ml glutathione
2mM L -glutamine +MEM non-essential amino acids
2mM L-glutamine + non-essential amino acids +1 mM sodium pyruvate + 10ug/ml human insulin
.005 mg/ml insulin, .01 mg/ml transferrin, 30nM sodium selenite, 10 nM hydrocortisone, 2 mM L-glutamine
4 mM L-glutamine, 1 mM sodium puruvate, 0.4 ug/ml hydrocortisone, 10 ng/ml EGF
2mM L- glutamine+1x non-essential amino acids+1mM sodium pyruvate
10% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10ng/ml GM-CSF
1L-3 ( 10ng/ml) or GM-CSF(10ng/ml) or 10-20% vol conditioned medium of cell line 5637(DSM-ACC 35)
1mM sodium pyruvate + insulin-transferrin-selentite medium supplement