RNA-seq Analysis 2021

Welcome

Welcome to Informatics for RNA-seq Analysis 2021.

The course schedule can be found here

Meet your faculty here

Pre-Workshop Preparation

Pre-work including programs to install can be found here

AWS Module

Connecting and properly using a cloud computing cluster at the CBW here


Class Photo

comming soon


Day 1

Welcome

Rachade/Francis

Introductions

Module 1 - Intro to RNAseq course/concepts

Malachi Griffith

Intro to RNA-seq Lecture

FASTA_FASTQ_GTF mini lecture

Indexing mini lecture

Module 1 Lab

Log into AWS

Installation

Reference Genomes

Annotations

Indexing

Data

Data QC

Module 2 - RNA alignment concepts and file formats

Obi Griffith

Alignment mini lecture

Alignment vs Assembly vs kmer mini lecture

SAM BAM BED mini lecture

IGV Tutorial lecture

Alignment QC mini lecture


Day 2

Module 2 Lab

Adapter Trim

Alignment

IGV

Alignment Visualization

Alignment QC

Module 3 - RNA Expression and Differential Expression

Obi Griffith

Abundance Estimation mini lecture

HTSeq mini lecture

Differential Expression mini lecture


Day 3

Module 3 Lab

Expression

Differential Expression

DE Visualization

Batch Correction

DE Pathway Analysis

Module 4 - Alignment Free Analysis

Malachi Griffith

Alignment Free - Kallisto mini lecture

Module 4 Lab

lab

additional resources

rnabio.org