Informatics for RNA-Seq Analysis 2020

Do these before coming to the workshop:

1) R tutorial: You are expected to be familiar with R before the workshop. If you are unfamiliar with R, here is a suggested tutorial and command reference. Using R installed on your computer please work through the tutorial.

• The GenViz Intro R tutorial
• The R command cheat sheet

2) Command line tutorial: You are expected to be familiar with basic command line usage before the workshop. If you are unfamiliar with the command line, here is a suggested tutorial. If you have a Mac computer you can use the “Terminal” application to complete this tutorial. If you have access to a linux server at your institution you could do it there. If neither of those is available you can try a free online termline application that runs in your browser such as COCALC.

• UNIX Intro Tutorial
• Unix Cheat sheet

3) Reading materials: Please read these two articles before coming to the workshop:

• RNA-seq data fundamentals. RNA Sequencing: The Teenage Years.

• RNA-seq analysis and statistics fundamentals. RNA Sequencing Data: Hitchhiker’s Guide to Expression Analysis.

Optional readings:

These are just two recently published examples that we find particularly good. We have collected a much more extensive list of resources that should be aware of below. Simply take a look at these and note for future reference.

• RNA-seq resources