Informatics for RNA-Seq Analysis
Welcome
Welcome to Informatics for RNA-Seq Analysis 2017.
The course schedule can be found here.
Pre-readings, pre-work, and laptop setup instructions can be found here
If you have questions or comments, visit the workshop Q&A forum.
Class Photo
Day 1
Day 1 and Day 2 refer to content in the RNA-Seq wiki.
Welcome
Ann Meyer
Introduction to Cloud Computing
Obi Griffith
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We have set up 30 instances on the Amazon cloud - one for each student. In order to log in to your instance, you will need a security certificate. If you plan on using Linux or Mac OS X, please download this certificate. Otherwise if you plan on using Windows (with Putty), please download this certificate.
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Detail instructions can be found here.
Module 1: Introduction to RNA Sequencing Analysis
Malachi Griffith
Lab Practical
Module 2: RNA-seq Alignment and Visualization
Fouad Yousif
Lab Practical
Integrated Assignment
Fouad Yousif
Paper: Recurrent chimeric RNAs enriched in human prostate cancer identified by deep sequencing
Day 2
Module 3: Expression and Differential Expression
Obi Griffith
Lab Practical
Module 4: Reference Free Alignment
Malachi Griffith
Module 5: Isoform Discovery and Alternative Expression
Malachi Griffith
Lab Practical
Integrated Assignment
Keeping Up-to-date with RNA-Seq Analysis Developments
For additional resources, tutorials, future directions, and more please refer to the RNA-seq wiki
Day 3
Module 6: Genome-Free De Novo Transcriptome Assembly
Brian Haas
Module 7: Functional Annotation and Analysis of Transcripts
Brian Haas
Thank you for attending the Informatics and RNA-Seq Analysis workshop! Help us make this workshop better by filling out our survey