Single Cell RNA-seq Analysis
I Introduction
Workshop Info
Pre-work
Class Photo
Winnipeg
Toronto
Schedule
Meet Your Faculty
Data and Compute Setup
Module 1
Lecture
Lab
scRNA-seq Analysis: datastructures
Module 2
Lecture
Lab
Reading single-cell data
QC with the
Seurat
package.
Mitochondrial Proportion
Identifying Doublets (DoubletFinder)
Module 3
Lecture
Lab
Normalizing scRNA-seq Datasets
Module 4
Lecture
Lab
Data Visualization and Dimensionality Reduction
tSNE
Destiny & DiffusionMaps
Overview
Module 5
Lecture
Lab
Preprocessing
Clustering
Cell-cell graph
Clustering
Finding optimal clusters
Annotating Clusters
Reference based annotation
Differential Expression
Pseudobulks
EdgeR
Overview
Module 6
Lecture
Lab
Harmony, 3’ vs 5’ 10k PBMC
LIGER, 3’ vs 5’ 10k PBMC
Sponsors
Single Cell RNA-seq Analysis 2026
Data and Compute Setup
Course data downloads
Coming soon!
Compute setup
Coming soon!
###(PART) Modules {-}