Welcome to Informatics for RNA-Seq Analysis 2019.
The course schedule can be found here.
Meet your faculty here
We are using Slack for discussion. Join the Slack channel.
CLASS MATERIALS: The full course notes are available as a single PDF.
Laptop Setup Instructions
Instructions to setup your laptop can be found here.
Pre-readings and pre-work can be found here here.
It is in your best interest to complete these before the workshop.
Day 1 and Day 2 refer to content on rnabio.org.
Module 1: Introduction to Cloud Computing
- We have set up instances on the Amazon cloud - one for each student. In order to log in to your instance, you will need a security certificate. The certificates will be uploaded to Slack. If you plan on using Linux or Mac OS X, Please use CBWRNA.pem; Otherwise if you plan on using Windows (with Putty), please use CBWRNA.ppk.
Module 2: Introduction to RNA Sequencing Analysis
Module 2 Lab Practical
Module 3: RNA-seq Alignment and Visualization
Module 3: RNA-seq Alignment and Visualization Lab Practical
Megan Richters and Kelsy Cotto
Module 4: Expression and Differential Expression
Module 4 Lab
Module 5: Reference Free Alignment
Module 6: Isoform Discovery and Alternative Expression
Keeping Up-to-date with RNA-Seq Analysis Developments
For additional resources, tutorials, future directions, and more please refer to the RNA-seq wiki
Module 7: Genome Guided and Genome-Free Transcriptome Assembly
Module 8: Functional Annotation and Analysis of Transcripts
Post workshop content for data sets, AWS image, tools and install instructions can be found here
Additional Optional Resources
Additional resources can be found on the RNA-Seq wiki.
Thank you for attending the Informatics for RNA-Seq Analysis workshop! Help us make this workshop better by filling out our survey